Investigation the effect of zinc oxide and nickel nano- particles on visceral leishmaniasis in-vitro

Abstract

Abstract:

Leishmaniasis is a widespread ailment in the Iraq, encompassing both cutaneous and visceral. The present study was conducted to investigate the anti-leishmanial activity of zinc oxide nanoparticles (ZnO NPs) compared to nickel nanoparticles which both biosynthesized by using clove extract as a reducing agent for particle manufacturing. Bio-based zinc oxide nanoparticles and nickel nanoparticles with an average particle size of less than 100 nanometers (nm) were produced and characterized using by UV–Vis spectroscopy, Scanning electron microscope, FTIR analysis and X-rays diffraction. The antileishmanial activities were investigated through (MTT) 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (cell viability assay. After 24 h of exposure with six concentrations nanoparticles (ZnO and Ni NPs), a concentration-dependent growth inhibition was observed. The IC50 values were determined against promastigotes of L. donovani as 59.06 µg/ml for ZnO NPs and 39,55 µg/ml for NiNPs, with observing statistically significant differences (p < 0.05). The results indicated that ZnO NPs and NiNPs had noticeable effect on the inhibition ratio and viability of the L. donovani promastigotes in a dose-dependent manner under laboratory conditions. recommend study Based on the present results, it has been concluded that ZnO NPs had little effect on L. donvani at the same concentrations in vitro. On the other hand, there is a direct destructive effect of nickel on different forms (promastigotes) of Leishmania donvani parasite, also, the destruction of parasites increases with concentrations of nickel NPs used, and the best concentration was 160 μg/ml after 24hr.

 Aims:

1- Produce synthesized biological nanoparticles including (zinic oxide and nickel) from clove extract.

2- Study the effect of zinc oxide on Leishmania donavani.

3- Study the effect of nickel on Leishmania donavani.

4-Investigate and compare the impact of these nano- particles on viability of promastigote in MTT assay in in-vitro condition.