Antimicrobial effect of Capsaicin extracted from Capsicum annuum on HWP1 gene expression of Candida albicans.

Abstract

Background: Cutaneous lesions of candidiasis in dogs are characterized by erythematous moist erosions with an irregular contour and there may be alopecia, crusting, ulceration and edema. Therefore, the skin scrapings and skin biopsy are the main methods for cutaneous candidiasis diagnosis in dogs.Protein agglutinin-like sequence proteins (HWP1) are examples of adhesion proteins which activate with encoded many targeted genes like (HWP1) . HWP1 gene which are involved in C. albicans on host cells in cutaneous Candidiasis. Capsaicin is structure is extracted from Capsicum annuum L., it was showed that active for treatment of different microbial skin problems such as cutaneous candidiasis.Objective: The aim of this study was to detect the effectiveness of capsaicin against on the biofilm gene expression () for Can HWP1dida albicansisolated from canine cutaneous Candidiasis. Material and methods:A total of 100 skin samples from German Shepherd-K9 dogs (Canis lupus L.) which infected with cutaneous candidiasis. All of the isolated C. albicans were examined under a microscope, on a larger scale, and using biochemistry. screening and purification methods were used to look into the detection of capsaicin from Capsicum annuum L.. The expression of (HWP1) gene before and after treatment with capsaicin was compared using reverse transcription quantitative polymerase chain reaction (RT-qPCR). Results: The agar wells diffusion technique was the most effective, according to the results. The results show that folding of this gene was reduced after capsaicin treatment which means that capsaicin inhibits the gene expression of biofilm formation of C. albicans. Conclusions: Our conclusions were the gene expression showed that the levels of gene HWP1were up-regulated in biofilm formation isolates with highest expression folding 1.00 before capsaicin treatment and the expression was reduced after treatment.